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KMID : 0617319910010010078
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Journal of Pharmacetical Sceiences Ewha Womans University
1991 Volume.1 No. 1 p.78 ~ p.85
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Identification of Intermediate of the Metabolism of Methylthioadenosine by Cell-free Extracts of Actinoplanes ferrugineus
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Abstract
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To identify intermediate(s) during the conversion of methylthioadenosine to methionine by cell-free extracts of Actinoplanes ferrugineus, a compound was isolated from the anaerobic incubation mixture of cell-free extracts and CH_3-[^14C]-methylthioadenosine, followed by 0.01 N-HCI hycrolysis. The compound was characterized through alkaline phosphatase treatment, NaBH_4 reduction, and NaBH_4 reduction-periodate oxidation reaction. The intermediate seemed to be 1-phospho-5-methylthio-5-deoxyribulose.
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